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Fusing an exonuclease with Cas9 enhances homologous recombination in Pichia pastoris

BACKGROUND: The methylotrophic yeast Pichia pastoris is considered as an ideal host for the production of recombinant proteins and chemicals. However, low homologous recombination (HR) efficiency hinders its precise and extensive genetic manipulation. To enhance the homology-directed repair over non...

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Detalles Bibliográficos
Autores principales:	Zhang, Kun, Duan, Xingpeng, Cai, Peng, Gao, Linhui, Wu, Xiaoyan, Yao, Lun, Zhou, Yongjin J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9450370/ https://www.ncbi.nlm.nih.gov/pubmed/36071435 http://dx.doi.org/10.1186/s12934-022-01908-z

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