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The application of CRISPR /Cas mediated gene editing in synthetic biology: Challenges and optimizations
Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated enzymes (Cas) is a simple and convenient genome editing tool that has been used in various cell factories and emerging synthetic biology in the recent past. However, several problems, including off-target effects,...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Frontiers Media S.A.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9452635/ https://www.ncbi.nlm.nih.gov/pubmed/36091445 http://dx.doi.org/10.3389/fbioe.2022.890155 |