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Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts

Algal biomass (AB) is prospective source of valuable compounds, however, Baltic Sea macroalgae have some challenges, because of their high microbial and chemical contamination. These problems can be solved, by using appropriate technologies for AG pre-treatment. The aim of this study was to evaluate...

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Autores principales: Tolpeznikaite, Ernesta, Starkute, Vytaute, Zokaityte, Egle, Ruzauskas, Modestas, Pilkaityte, Renata, Viskelis, Pranas, Urbonaviciene, Dalia, Ruibys, Romas, Rocha, João M., Bartkiene, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453264/
https://www.ncbi.nlm.nih.gov/pubmed/36091232
http://dx.doi.org/10.3389/fnut.2022.990274
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author Tolpeznikaite, Ernesta
Starkute, Vytaute
Zokaityte, Egle
Ruzauskas, Modestas
Pilkaityte, Renata
Viskelis, Pranas
Urbonaviciene, Dalia
Ruibys, Romas
Rocha, João M.
Bartkiene, Elena
author_facet Tolpeznikaite, Ernesta
Starkute, Vytaute
Zokaityte, Egle
Ruzauskas, Modestas
Pilkaityte, Renata
Viskelis, Pranas
Urbonaviciene, Dalia
Ruibys, Romas
Rocha, João M.
Bartkiene, Elena
author_sort Tolpeznikaite, Ernesta
collection PubMed
description Algal biomass (AB) is prospective source of valuable compounds, however, Baltic Sea macroalgae have some challenges, because of their high microbial and chemical contamination. These problems can be solved, by using appropriate technologies for AG pre-treatment. The aim of this study was to evaluate the influence of two pre-treatments, solid-state fermentation with the Lactiplantibacillus plantarum LUHS135 and ultrasonication, on the antioxidant and antimicrobial characteristics of macro- (Cladophora rupestris, Cladophora glomerata, Furcellaria lumbricalis, Ulva intestinalis) and Spirulina (Arthrospira platensis) extracts. Also, combinations of extracts and LUHS135 were developed and their characteristics were evaluated. The total phenolic compound content was determined from the calibration curve and expressed in mg of gallic acid equivalents; antioxidant activity was measured by a Trolox equivalent antioxidant capacity assay using the DPPH(•) (1,1-diphenyl-2-picrylhydrazyl), ABTS(•+) 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (Ferric Reducing Ability of Plasma) discoloration methods. Antimicrobial activity was measured by using agar well diffusion assay and in a liquid medium. The highest DPPH(•) and ABTS(•+) was shown by C.rupestris and F.lumbricalis extract × LUHS135 combinations, the highest FRAP - by non-pretreated C.rupestris and F.lumbricalis extract × LUHS135 combinations. Ultrasonicated samples inhibited four out of seven tested pathogens. Finally, the tested pre-treatments showed good perspectives and can be recommended for AB valorization.
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spelling pubmed-94532642022-09-09 Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts Tolpeznikaite, Ernesta Starkute, Vytaute Zokaityte, Egle Ruzauskas, Modestas Pilkaityte, Renata Viskelis, Pranas Urbonaviciene, Dalia Ruibys, Romas Rocha, João M. Bartkiene, Elena Front Nutr Nutrition Algal biomass (AB) is prospective source of valuable compounds, however, Baltic Sea macroalgae have some challenges, because of their high microbial and chemical contamination. These problems can be solved, by using appropriate technologies for AG pre-treatment. The aim of this study was to evaluate the influence of two pre-treatments, solid-state fermentation with the Lactiplantibacillus plantarum LUHS135 and ultrasonication, on the antioxidant and antimicrobial characteristics of macro- (Cladophora rupestris, Cladophora glomerata, Furcellaria lumbricalis, Ulva intestinalis) and Spirulina (Arthrospira platensis) extracts. Also, combinations of extracts and LUHS135 were developed and their characteristics were evaluated. The total phenolic compound content was determined from the calibration curve and expressed in mg of gallic acid equivalents; antioxidant activity was measured by a Trolox equivalent antioxidant capacity assay using the DPPH(•) (1,1-diphenyl-2-picrylhydrazyl), ABTS(•+) 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid), FRAP (Ferric Reducing Ability of Plasma) discoloration methods. Antimicrobial activity was measured by using agar well diffusion assay and in a liquid medium. The highest DPPH(•) and ABTS(•+) was shown by C.rupestris and F.lumbricalis extract × LUHS135 combinations, the highest FRAP - by non-pretreated C.rupestris and F.lumbricalis extract × LUHS135 combinations. Ultrasonicated samples inhibited four out of seven tested pathogens. Finally, the tested pre-treatments showed good perspectives and can be recommended for AB valorization. Frontiers Media S.A. 2022-08-26 /pmc/articles/PMC9453264/ /pubmed/36091232 http://dx.doi.org/10.3389/fnut.2022.990274 Text en Copyright © 2022 Tolpeznikaite, Starkute, Zokaityte, Ruzauskas, Pilkaityte, Viskelis, Urbonaviciene, Ruibys, Rocha and Bartkiene. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Tolpeznikaite, Ernesta
Starkute, Vytaute
Zokaityte, Egle
Ruzauskas, Modestas
Pilkaityte, Renata
Viskelis, Pranas
Urbonaviciene, Dalia
Ruibys, Romas
Rocha, João M.
Bartkiene, Elena
Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
title Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
title_full Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
title_fullStr Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
title_full_unstemmed Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
title_short Effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
title_sort effect of solid-state fermentation and ultrasonication processes on antimicrobial and antioxidant properties of algae extracts
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9453264/
https://www.ncbi.nlm.nih.gov/pubmed/36091232
http://dx.doi.org/10.3389/fnut.2022.990274
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