Applications of CRISPR/Cas13-Based RNA Editing in Plants

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement. Among the CRIS...

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Autores principales: Kavuri, Naga Rajitha, Ramasamy, Manikandan, Qi, Yiping, Mandadi, Kranthi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454418/
https://www.ncbi.nlm.nih.gov/pubmed/36078073
http://dx.doi.org/10.3390/cells11172665
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author Kavuri, Naga Rajitha
Ramasamy, Manikandan
Qi, Yiping
Mandadi, Kranthi
author_facet Kavuri, Naga Rajitha
Ramasamy, Manikandan
Qi, Yiping
Mandadi, Kranthi
author_sort Kavuri, Naga Rajitha
collection PubMed
description The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement. Among the CRISPR/Cas systems, Cas9 is one of the widely used nucleases for DNA modifications, but manipulation of RNA at the post-transcriptional level is limited. The recently identified type VI CRISPR/Cas systems provide a platform for precise RNA manipulation without permanent changes to the genome. Several studies reported efficient application of Cas13 in RNA studies, such as viral interference, RNA knockdown, and RNA detection in various organisms. Cas13 was also used to produce virus resistance in plants, as most plant viruses are RNA viruses. However, the application of CRISPR/Cas13 to studies of plant RNA biology is still in its infancy. This review discusses the current and prospective applications of CRISPR/Cas13-based RNA editing technologies in plants.
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spelling pubmed-94544182022-09-09 Applications of CRISPR/Cas13-Based RNA Editing in Plants Kavuri, Naga Rajitha Ramasamy, Manikandan Qi, Yiping Mandadi, Kranthi Cells Review The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement. Among the CRISPR/Cas systems, Cas9 is one of the widely used nucleases for DNA modifications, but manipulation of RNA at the post-transcriptional level is limited. The recently identified type VI CRISPR/Cas systems provide a platform for precise RNA manipulation without permanent changes to the genome. Several studies reported efficient application of Cas13 in RNA studies, such as viral interference, RNA knockdown, and RNA detection in various organisms. Cas13 was also used to produce virus resistance in plants, as most plant viruses are RNA viruses. However, the application of CRISPR/Cas13 to studies of plant RNA biology is still in its infancy. This review discusses the current and prospective applications of CRISPR/Cas13-based RNA editing technologies in plants. MDPI 2022-08-27 /pmc/articles/PMC9454418/ /pubmed/36078073 http://dx.doi.org/10.3390/cells11172665 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kavuri, Naga Rajitha
Ramasamy, Manikandan
Qi, Yiping
Mandadi, Kranthi
Applications of CRISPR/Cas13-Based RNA Editing in Plants
title Applications of CRISPR/Cas13-Based RNA Editing in Plants
title_full Applications of CRISPR/Cas13-Based RNA Editing in Plants
title_fullStr Applications of CRISPR/Cas13-Based RNA Editing in Plants
title_full_unstemmed Applications of CRISPR/Cas13-Based RNA Editing in Plants
title_short Applications of CRISPR/Cas13-Based RNA Editing in Plants
title_sort applications of crispr/cas13-based rna editing in plants
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9454418/
https://www.ncbi.nlm.nih.gov/pubmed/36078073
http://dx.doi.org/10.3390/cells11172665
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