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The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme

In this study, the protein stability of hen egg-white lysozymes (HEWL) by Fe(3)O(4) and Fe(3)O(4)-coated trehalose (Fe(3)O(4)@Tre) magnetic nanoparticles (NPs) is investigated. For this purpose, the co-precipitation method was used to synthesize magnetic NPs. The synthesized NPs were characterized b...

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Autores principales: Lajmorak, Asma, Seyyed Ebrahimi, Seyyed Ali, Yazdian, Fatemeh, Lalegani, Zahra, Hamawandi, Bejan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456156/
https://www.ncbi.nlm.nih.gov/pubmed/36077055
http://dx.doi.org/10.3390/ijms23179657
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author Lajmorak, Asma
Seyyed Ebrahimi, Seyyed Ali
Yazdian, Fatemeh
Lalegani, Zahra
Hamawandi, Bejan
author_facet Lajmorak, Asma
Seyyed Ebrahimi, Seyyed Ali
Yazdian, Fatemeh
Lalegani, Zahra
Hamawandi, Bejan
author_sort Lajmorak, Asma
collection PubMed
description In this study, the protein stability of hen egg-white lysozymes (HEWL) by Fe(3)O(4) and Fe(3)O(4)-coated trehalose (Fe(3)O(4)@Tre) magnetic nanoparticles (NPs) is investigated. For this purpose, the co-precipitation method was used to synthesize magnetic NPs. The synthesized NPs were characterized by XRD, FT-IR spectroscopy, FE-SEM, and VSM analysis. In addition, the stability of HEWLs exposed to different NP concentrations in the range of 0.001–0.1 mg mL(−1) was investigated by circular dichroism (CD) spectroscopy, fluorescence, and UV-Vis analysis. Based on the results, in the NP concentration range of 0.001–0.04 mg mL(−1) the protein structure is more stable, and this range was identified as the range of kosmotropic concentration. The helicity was measured at two concentration points of 0.02 and 0.1 mg mL(−1). According to the results, the α-helix at 0.02 mg mL(−1) of Fe(3)O(4) and Fe(3)O(4)@Tre was increased from 35.5% for native protein to 37.7% and 38.7%, respectively. The helicity decreased to 36.1% and 37.4%, respectively, with increasing the concentration of Fe(3)O(4) and Fe(3)O(4)@Tre to 0.1 mg mL(−1). The formation of hydrated water shells around protein molecules occurred by using Fe(3)O(4)@Tre NPs. Hence, it can be concluded that the trehalose as a functional group along with magnetic NPs can improve the stability of proteins in biological environments.
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spelling pubmed-94561562022-09-09 The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme Lajmorak, Asma Seyyed Ebrahimi, Seyyed Ali Yazdian, Fatemeh Lalegani, Zahra Hamawandi, Bejan Int J Mol Sci Article In this study, the protein stability of hen egg-white lysozymes (HEWL) by Fe(3)O(4) and Fe(3)O(4)-coated trehalose (Fe(3)O(4)@Tre) magnetic nanoparticles (NPs) is investigated. For this purpose, the co-precipitation method was used to synthesize magnetic NPs. The synthesized NPs were characterized by XRD, FT-IR spectroscopy, FE-SEM, and VSM analysis. In addition, the stability of HEWLs exposed to different NP concentrations in the range of 0.001–0.1 mg mL(−1) was investigated by circular dichroism (CD) spectroscopy, fluorescence, and UV-Vis analysis. Based on the results, in the NP concentration range of 0.001–0.04 mg mL(−1) the protein structure is more stable, and this range was identified as the range of kosmotropic concentration. The helicity was measured at two concentration points of 0.02 and 0.1 mg mL(−1). According to the results, the α-helix at 0.02 mg mL(−1) of Fe(3)O(4) and Fe(3)O(4)@Tre was increased from 35.5% for native protein to 37.7% and 38.7%, respectively. The helicity decreased to 36.1% and 37.4%, respectively, with increasing the concentration of Fe(3)O(4) and Fe(3)O(4)@Tre to 0.1 mg mL(−1). The formation of hydrated water shells around protein molecules occurred by using Fe(3)O(4)@Tre NPs. Hence, it can be concluded that the trehalose as a functional group along with magnetic NPs can improve the stability of proteins in biological environments. MDPI 2022-08-25 /pmc/articles/PMC9456156/ /pubmed/36077055 http://dx.doi.org/10.3390/ijms23179657 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lajmorak, Asma
Seyyed Ebrahimi, Seyyed Ali
Yazdian, Fatemeh
Lalegani, Zahra
Hamawandi, Bejan
The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme
title The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme
title_full The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme
title_fullStr The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme
title_full_unstemmed The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme
title_short The Effect of Trehalose Coating for Magnetite Nanoparticles on Stability of Egg White Lysozyme
title_sort effect of trehalose coating for magnetite nanoparticles on stability of egg white lysozyme
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9456156/
https://www.ncbi.nlm.nih.gov/pubmed/36077055
http://dx.doi.org/10.3390/ijms23179657
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