Sequence-dependent mechanochemical coupling of helicase translocation and unwinding at single-nucleotide resolution

We used single-molecule picometer-resolution nanopore tweezers (SPRNT) to resolve the millisecond single-nucleotide steps of superfamily 1 helicase PcrA as it translocates on, or unwinds, several kilobase-long DNA molecules. We recorded more than two million enzyme steps under various assisting and...

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Detalles Bibliográficos
Autores principales: Laszlo, Andrew H., Craig, Jonathan M., Gavrilov, Momčilo, Tippana, Ramreddy, Nova, Ian C., Huang, Jesse R., Kim, Hwanhee C., Abell, Sarah J., deCampos-Stairiker, Mallory, Mount, Jonathan W., Bowman, Jasmine L., Baker, Katherine S., Higinbotham, Hugh, Bobrovnikov, Dmitriy, Ha, Taekjip, Gundlach, Jens H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457475/
https://www.ncbi.nlm.nih.gov/pubmed/36037333
http://dx.doi.org/10.1073/pnas.2202489119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457475/
https://www.ncbi.nlm.nih.gov/pubmed/36037333
http://dx.doi.org/10.1073/pnas.2202489119

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