Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants

Exosomes play a key role in virus exocytosis and transmission. The exportin family is usually responsible for cargo nucleocytoplasmic trafficking, and they are frequently found in exosomes. However, the function of exportins sorted in exosomes remains unknown. Here, we successfully isolated “cup hol...

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Autores principales: Lu, Hong, Zhu, Jiaming, Yu, Jinting, Li, Qiong, Luo, Lan, Cui, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457540/
https://www.ncbi.nlm.nih.gov/pubmed/36037368
http://dx.doi.org/10.1073/pnas.2207848119
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author Lu, Hong
Zhu, Jiaming
Yu, Jinting
Li, Qiong
Luo, Lan
Cui, Feng
author_facet Lu, Hong
Zhu, Jiaming
Yu, Jinting
Li, Qiong
Luo, Lan
Cui, Feng
author_sort Lu, Hong
collection PubMed
description Exosomes play a key role in virus exocytosis and transmission. The exportin family is usually responsible for cargo nucleocytoplasmic trafficking, and they are frequently found in exosomes. However, the function of exportins sorted in exosomes remains unknown. Here, we successfully isolated “cup holder”-like exosomes from the saliva of ∼30,000 small brown planthoppers, which are vectors of rice stripe virus (RSV). RSV virions were packed in comparatively large exosomes. Four viral genomic RNAs at a certain ratio were identified in the saliva exosomes. The virions contained in the saliva exosomes were capable of replicating and causing disease in rice plants. Interference with each phase of the insect exosome system affected the transmission of RSV from the insect vectors to rice plants. Fragmented exportin 6 was coimmunoprecipitated with viral nucleocapsid protein in saliva and sorted to exosomes via interactions with the cargo sorting protein VPS37a. When the expression of exportin 6 was knocked down, the amounts of RSV secreted in saliva and rice plants were reduced by 60% and 74%, respectively. These results showed that exportin 6 acted as a vehicle for transporting RSV into exosomes to overcome the barrier of insect salivary glands for horizontal transmission. Exportin 6 would represent an ideal target that could be manipulated to control the outbreak of insect-borne viruses in the future.
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spelling pubmed-94575402023-03-01 Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants Lu, Hong Zhu, Jiaming Yu, Jinting Li, Qiong Luo, Lan Cui, Feng Proc Natl Acad Sci U S A Biological Sciences Exosomes play a key role in virus exocytosis and transmission. The exportin family is usually responsible for cargo nucleocytoplasmic trafficking, and they are frequently found in exosomes. However, the function of exportins sorted in exosomes remains unknown. Here, we successfully isolated “cup holder”-like exosomes from the saliva of ∼30,000 small brown planthoppers, which are vectors of rice stripe virus (RSV). RSV virions were packed in comparatively large exosomes. Four viral genomic RNAs at a certain ratio were identified in the saliva exosomes. The virions contained in the saliva exosomes were capable of replicating and causing disease in rice plants. Interference with each phase of the insect exosome system affected the transmission of RSV from the insect vectors to rice plants. Fragmented exportin 6 was coimmunoprecipitated with viral nucleocapsid protein in saliva and sorted to exosomes via interactions with the cargo sorting protein VPS37a. When the expression of exportin 6 was knocked down, the amounts of RSV secreted in saliva and rice plants were reduced by 60% and 74%, respectively. These results showed that exportin 6 acted as a vehicle for transporting RSV into exosomes to overcome the barrier of insect salivary glands for horizontal transmission. Exportin 6 would represent an ideal target that could be manipulated to control the outbreak of insect-borne viruses in the future. National Academy of Sciences 2022-08-29 2022-09-06 /pmc/articles/PMC9457540/ /pubmed/36037368 http://dx.doi.org/10.1073/pnas.2207848119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) .
spellingShingle Biological Sciences
Lu, Hong
Zhu, Jiaming
Yu, Jinting
Li, Qiong
Luo, Lan
Cui, Feng
Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
title Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
title_full Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
title_fullStr Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
title_full_unstemmed Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
title_short Key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
title_sort key role of exportin 6 in exosome-mediated viral transmission from insect vectors to plants
topic Biological Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9457540/
https://www.ncbi.nlm.nih.gov/pubmed/36037368
http://dx.doi.org/10.1073/pnas.2207848119
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