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Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes
Foxp3(+) regulatory T cells (Tregs) are critical mediators of peripheral tolerance and immune homeostasis and exert tissue-specific functions. In many nonlymphoid tissues, Tregs show enriched expression of the IL-33 receptor ST2. Through comprehensive profiling of murine ST2(+) and ST2(-) Tregs, we...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460833/ https://www.ncbi.nlm.nih.gov/pubmed/36093048 http://dx.doi.org/10.1016/j.isci.2022.104998 |
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author | Spath, Sabine Roan, Florence Presnell, Scott R. Höllbacher, Barbara Ziegler, Steven F. |
author_facet | Spath, Sabine Roan, Florence Presnell, Scott R. Höllbacher, Barbara Ziegler, Steven F. |
author_sort | Spath, Sabine |
collection | PubMed |
description | Foxp3(+) regulatory T cells (Tregs) are critical mediators of peripheral tolerance and immune homeostasis and exert tissue-specific functions. In many nonlymphoid tissues, Tregs show enriched expression of the IL-33 receptor ST2. Through comprehensive profiling of murine ST2(+) and ST2(-) Tregs, we found that Treg transcriptomes and phenotypes formed a hierarchical relationship across tissues. Only a small core signature distinguished ST2(+) Tregs from ST2(-) Tregs across all tissues, and differences in transcriptional profiles were predominantly tissue-specific. We also identified unique, highly proliferative, circulating ST2(+) Tregs with high migratory potential. In adoptive transfers, both ST2(+) and ST2(-) Tregs seeded various host tissues and demonstrated plasticity in ST2 expression. Furthermore, Tregs from donor lungs were differentially recovered from host nonlymphoid tissues in an IL-33-dependent manner. In summary, our work identified tissue residency rather than ST2 expression as a primary driver of tissue Treg identity and highlights the unique, tissue-specific adaption of ST2(+) Tregs. |
format | Online Article Text |
id | pubmed-9460833 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-94608332022-09-10 Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes Spath, Sabine Roan, Florence Presnell, Scott R. Höllbacher, Barbara Ziegler, Steven F. iScience Article Foxp3(+) regulatory T cells (Tregs) are critical mediators of peripheral tolerance and immune homeostasis and exert tissue-specific functions. In many nonlymphoid tissues, Tregs show enriched expression of the IL-33 receptor ST2. Through comprehensive profiling of murine ST2(+) and ST2(-) Tregs, we found that Treg transcriptomes and phenotypes formed a hierarchical relationship across tissues. Only a small core signature distinguished ST2(+) Tregs from ST2(-) Tregs across all tissues, and differences in transcriptional profiles were predominantly tissue-specific. We also identified unique, highly proliferative, circulating ST2(+) Tregs with high migratory potential. In adoptive transfers, both ST2(+) and ST2(-) Tregs seeded various host tissues and demonstrated plasticity in ST2 expression. Furthermore, Tregs from donor lungs were differentially recovered from host nonlymphoid tissues in an IL-33-dependent manner. In summary, our work identified tissue residency rather than ST2 expression as a primary driver of tissue Treg identity and highlights the unique, tissue-specific adaption of ST2(+) Tregs. Elsevier 2022-08-24 /pmc/articles/PMC9460833/ /pubmed/36093048 http://dx.doi.org/10.1016/j.isci.2022.104998 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Spath, Sabine Roan, Florence Presnell, Scott R. Höllbacher, Barbara Ziegler, Steven F. Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes |
title | Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes |
title_full | Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes |
title_fullStr | Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes |
title_full_unstemmed | Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes |
title_short | Profiling of Tregs across tissues reveals plasticity in ST2 expression and hierarchies in tissue-specific phenotypes |
title_sort | profiling of tregs across tissues reveals plasticity in st2 expression and hierarchies in tissue-specific phenotypes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9460833/ https://www.ncbi.nlm.nih.gov/pubmed/36093048 http://dx.doi.org/10.1016/j.isci.2022.104998 |
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