CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA

The all-protein cytosine base editor DdCBE uses TALE proteins and a double-stranded DNA-specific cytidine deaminase (DddA) to mediate targeted C•G-to-T•A editing. To improve editing efficiency and overcome the strict TC sequence-context constraint of DddA, we used phage-assisted non-continuous and c...

Descripción completa

Detalles Bibliográficos
Autores principales: Mok, Beverly Y., Kotrys, Anna V., Raguram, Aditya, Huang, Tony P., Mootha, Vamsi K., Liu, David R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463067/
https://www.ncbi.nlm.nih.gov/pubmed/35379961
http://dx.doi.org/10.1038/s41587-022-01256-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463067/
https://www.ncbi.nlm.nih.gov/pubmed/35379961
http://dx.doi.org/10.1038/s41587-022-01256-8

Ejemplares similares