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CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA
The all-protein cytosine base editor DdCBE uses TALE proteins and a double-stranded DNA-specific cytidine deaminase (DddA) to mediate targeted C•G-to-T•A editing. To improve editing efficiency and overcome the strict TC sequence-context constraint of DddA, we used phage-assisted non-continuous and c...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group US
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463067/ https://www.ncbi.nlm.nih.gov/pubmed/35379961 http://dx.doi.org/10.1038/s41587-022-01256-8 |