Temperature dependent in vitro binding and release of target DNA by Cas9 enzyme

The CRISPR-associated protein 9 (Cas9) system has proven to be a powerful technology for genome editing in a wide variety of in vivo and in vitro applications. CRISPR–Cas9, when loaded with the guide RNA, cleaves the DNA at the target position as recognized by the guide RNA sequence. For successful...

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Detalles Bibliográficos
Autores principales: David, Serene Rose, Maheshwaram, Sumanth Kumar, Shet, Divya, Lakshminarayana, Mahesh B., Soni, Gautam V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463129/
https://www.ncbi.nlm.nih.gov/pubmed/36085316
http://dx.doi.org/10.1038/s41598-022-19485-x

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9463129/
https://www.ncbi.nlm.nih.gov/pubmed/36085316
http://dx.doi.org/10.1038/s41598-022-19485-x

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