Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system

Controlling the abundance of a protein of interest in vivo is crucial to study its function. Here, we provide a step-by-step protocol for generating genetically engineered mouse (GEM) models harboring a degradation tag (dTAG) fused to endogenous proteins to enable their degradation. We discuss consi...

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Detalles Bibliográficos
Autores principales: Abuhashem, Abderhman, Hadjantonakis, Anna-Katerina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467879/
https://www.ncbi.nlm.nih.gov/pubmed/36097386
http://dx.doi.org/10.1016/j.xpro.2022.101660

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467879/
https://www.ncbi.nlm.nih.gov/pubmed/36097386
http://dx.doi.org/10.1016/j.xpro.2022.101660

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