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Generation of knock-in degron tags for endogenous proteins in mice using the dTAG system
Controlling the abundance of a protein of interest in vivo is crucial to study its function. Here, we provide a step-by-step protocol for generating genetically engineered mouse (GEM) models harboring a degradation tag (dTAG) fused to endogenous proteins to enable their degradation. We discuss consi...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9467879/ https://www.ncbi.nlm.nih.gov/pubmed/36097386 http://dx.doi.org/10.1016/j.xpro.2022.101660 |