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Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas

OBJECTIVES: To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs. METHODS: Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoStri...

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Autores principales: Wang, Depeng, Gong, Jie, Zhang, Hui, Liu, Yulu, Sun, Nannan, Hao, Xiaomeng, Mu, Kun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469524/
https://www.ncbi.nlm.nih.gov/pubmed/36096860
http://dx.doi.org/10.1186/s13000-022-01250-3
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author Wang, Depeng
Gong, Jie
Zhang, Hui
Liu, Yulu
Sun, Nannan
Hao, Xiaomeng
Mu, Kun
author_facet Wang, Depeng
Gong, Jie
Zhang, Hui
Liu, Yulu
Sun, Nannan
Hao, Xiaomeng
Mu, Kun
author_sort Wang, Depeng
collection PubMed
description OBJECTIVES: To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs. METHODS: Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoString and sequencing techniques for 30 MBs. RESULTS: By genetic defining, 3 MBs were WNT-activated, 11 were SHH-activated, 3 were in Group 3 and 13 in Group 4 respectively. Nuclear LEF-1 staining was found in 8 MBs using immunohistochemical method. Three out of 8 showed diffuse and strong nuclear LEF-1 staining which were proved to be WNT-activated genetically, while the other 5 MBs with focal staining were SHH-activated genetically. The expression of LEF-1 protein was significantly correlated with genetically defined WNT-activated MBs (P < 0.0001). We also found focal nuclear β-catenin expression ( less than 1% of tumor cells) in 5 MBs. LEF-1 positivity was significantly correlated nuclear β-catenin expression (p < 0.001). CONCLUSIONS: Immunohistochemical staining of LEF-1 can be used as a supplement for β-catenin to diagnosis WNT-activated Medulloblastomas, when β-catenin is difficult to recognize for its cytoplasm/membrane staining background. Diffuse nuclear staining of LEF-1 indicates WNT-activated MB.
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spelling pubmed-94695242022-09-14 Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas Wang, Depeng Gong, Jie Zhang, Hui Liu, Yulu Sun, Nannan Hao, Xiaomeng Mu, Kun Diagn Pathol Research OBJECTIVES: To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs. METHODS: Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoString and sequencing techniques for 30 MBs. RESULTS: By genetic defining, 3 MBs were WNT-activated, 11 were SHH-activated, 3 were in Group 3 and 13 in Group 4 respectively. Nuclear LEF-1 staining was found in 8 MBs using immunohistochemical method. Three out of 8 showed diffuse and strong nuclear LEF-1 staining which were proved to be WNT-activated genetically, while the other 5 MBs with focal staining were SHH-activated genetically. The expression of LEF-1 protein was significantly correlated with genetically defined WNT-activated MBs (P < 0.0001). We also found focal nuclear β-catenin expression ( less than 1% of tumor cells) in 5 MBs. LEF-1 positivity was significantly correlated nuclear β-catenin expression (p < 0.001). CONCLUSIONS: Immunohistochemical staining of LEF-1 can be used as a supplement for β-catenin to diagnosis WNT-activated Medulloblastomas, when β-catenin is difficult to recognize for its cytoplasm/membrane staining background. Diffuse nuclear staining of LEF-1 indicates WNT-activated MB. BioMed Central 2022-09-13 /pmc/articles/PMC9469524/ /pubmed/36096860 http://dx.doi.org/10.1186/s13000-022-01250-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Wang, Depeng
Gong, Jie
Zhang, Hui
Liu, Yulu
Sun, Nannan
Hao, Xiaomeng
Mu, Kun
Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
title Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
title_full Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
title_fullStr Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
title_full_unstemmed Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
title_short Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
title_sort immunohistochemical staining of lef-1 is a useful marker for distinguishing wnt-activated medulloblastomas
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469524/
https://www.ncbi.nlm.nih.gov/pubmed/36096860
http://dx.doi.org/10.1186/s13000-022-01250-3
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