Cargando…
Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas
OBJECTIVES: To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs. METHODS: Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoStri...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469524/ https://www.ncbi.nlm.nih.gov/pubmed/36096860 http://dx.doi.org/10.1186/s13000-022-01250-3 |
_version_ | 1784788662215507968 |
---|---|
author | Wang, Depeng Gong, Jie Zhang, Hui Liu, Yulu Sun, Nannan Hao, Xiaomeng Mu, Kun |
author_facet | Wang, Depeng Gong, Jie Zhang, Hui Liu, Yulu Sun, Nannan Hao, Xiaomeng Mu, Kun |
author_sort | Wang, Depeng |
collection | PubMed |
description | OBJECTIVES: To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs. METHODS: Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoString and sequencing techniques for 30 MBs. RESULTS: By genetic defining, 3 MBs were WNT-activated, 11 were SHH-activated, 3 were in Group 3 and 13 in Group 4 respectively. Nuclear LEF-1 staining was found in 8 MBs using immunohistochemical method. Three out of 8 showed diffuse and strong nuclear LEF-1 staining which were proved to be WNT-activated genetically, while the other 5 MBs with focal staining were SHH-activated genetically. The expression of LEF-1 protein was significantly correlated with genetically defined WNT-activated MBs (P < 0.0001). We also found focal nuclear β-catenin expression ( less than 1% of tumor cells) in 5 MBs. LEF-1 positivity was significantly correlated nuclear β-catenin expression (p < 0.001). CONCLUSIONS: Immunohistochemical staining of LEF-1 can be used as a supplement for β-catenin to diagnosis WNT-activated Medulloblastomas, when β-catenin is difficult to recognize for its cytoplasm/membrane staining background. Diffuse nuclear staining of LEF-1 indicates WNT-activated MB. |
format | Online Article Text |
id | pubmed-9469524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-94695242022-09-14 Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas Wang, Depeng Gong, Jie Zhang, Hui Liu, Yulu Sun, Nannan Hao, Xiaomeng Mu, Kun Diagn Pathol Research OBJECTIVES: To investigate lymphoid enhancer factor 1 (LEF-1) protein expression in medulloblastomas (MBs) and its correlation with molecular grouping of MBs. METHODS: Expressions of LEF-1 and β-catenin were detected by immunohistochemistry, and molecular grouping was performed based on the NanoString and sequencing techniques for 30 MBs. RESULTS: By genetic defining, 3 MBs were WNT-activated, 11 were SHH-activated, 3 were in Group 3 and 13 in Group 4 respectively. Nuclear LEF-1 staining was found in 8 MBs using immunohistochemical method. Three out of 8 showed diffuse and strong nuclear LEF-1 staining which were proved to be WNT-activated genetically, while the other 5 MBs with focal staining were SHH-activated genetically. The expression of LEF-1 protein was significantly correlated with genetically defined WNT-activated MBs (P < 0.0001). We also found focal nuclear β-catenin expression ( less than 1% of tumor cells) in 5 MBs. LEF-1 positivity was significantly correlated nuclear β-catenin expression (p < 0.001). CONCLUSIONS: Immunohistochemical staining of LEF-1 can be used as a supplement for β-catenin to diagnosis WNT-activated Medulloblastomas, when β-catenin is difficult to recognize for its cytoplasm/membrane staining background. Diffuse nuclear staining of LEF-1 indicates WNT-activated MB. BioMed Central 2022-09-13 /pmc/articles/PMC9469524/ /pubmed/36096860 http://dx.doi.org/10.1186/s13000-022-01250-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Wang, Depeng Gong, Jie Zhang, Hui Liu, Yulu Sun, Nannan Hao, Xiaomeng Mu, Kun Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas |
title | Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas |
title_full | Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas |
title_fullStr | Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas |
title_full_unstemmed | Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas |
title_short | Immunohistochemical staining of LEF-1 is a useful marker for distinguishing WNT-activated medulloblastomas |
title_sort | immunohistochemical staining of lef-1 is a useful marker for distinguishing wnt-activated medulloblastomas |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9469524/ https://www.ncbi.nlm.nih.gov/pubmed/36096860 http://dx.doi.org/10.1186/s13000-022-01250-3 |
work_keys_str_mv | AT wangdepeng immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas AT gongjie immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas AT zhanghui immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas AT liuyulu immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas AT sunnannan immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas AT haoxiaomeng immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas AT mukun immunohistochemicalstainingoflef1isausefulmarkerfordistinguishingwntactivatedmedulloblastomas |