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Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis
Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pat...
| Autores principales: | , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472670/ https://www.ncbi.nlm.nih.gov/pubmed/36119959 http://dx.doi.org/10.1093/ofid/ofac428 |
| _version_ | 1784789345870282752 |
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| author | Young, Bernadette C Bush, Stephen J Lipworth, Sam George, Sophie Dingle, Kate E Sanderson, Nick Brankin, Alice Walker, Timothy Sharma, Srilakshmi Leong, James Plaha, Puneet Hofer, Monika Chiodini, Peter Gottstein, B Furrer, Lavinia Crook, Derrick Brent, Andrew |
| author_facet | Young, Bernadette C Bush, Stephen J Lipworth, Sam George, Sophie Dingle, Kate E Sanderson, Nick Brankin, Alice Walker, Timothy Sharma, Srilakshmi Leong, James Plaha, Puneet Hofer, Monika Chiodini, Peter Gottstein, B Furrer, Lavinia Crook, Derrick Brent, Andrew |
| author_sort | Young, Bernadette C |
| collection | PubMed |
| description | Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts. |
| format | Online Article Text |
| id | pubmed-9472670 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2022 |
| publisher | Oxford University Press |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-94726702022-09-15 Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis Young, Bernadette C Bush, Stephen J Lipworth, Sam George, Sophie Dingle, Kate E Sanderson, Nick Brankin, Alice Walker, Timothy Sharma, Srilakshmi Leong, James Plaha, Puneet Hofer, Monika Chiodini, Peter Gottstein, B Furrer, Lavinia Crook, Derrick Brent, Andrew Open Forum Infect Dis Brief Report Microbes unculturable in vitro remain diagnostically challenging, dependent historically on clinical findings, histology, or targeted molecular detection. We applied whole-genome sequencing directly from tissue to diagnose infections with mycobacteria (leprosy) and parasites (coenurosis). Direct pathogen DNA sequencing provides flexible solutions to diagnosis of difficult pathogens in diverse contexts. Oxford University Press 2022-08-23 /pmc/articles/PMC9472670/ /pubmed/36119959 http://dx.doi.org/10.1093/ofid/ofac428 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
| spellingShingle | Brief Report Young, Bernadette C Bush, Stephen J Lipworth, Sam George, Sophie Dingle, Kate E Sanderson, Nick Brankin, Alice Walker, Timothy Sharma, Srilakshmi Leong, James Plaha, Puneet Hofer, Monika Chiodini, Peter Gottstein, B Furrer, Lavinia Crook, Derrick Brent, Andrew Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis |
| title | Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis |
| title_full | Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis |
| title_fullStr | Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis |
| title_full_unstemmed | Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis |
| title_short | Modern Solutions for Ancient Pathogens: Direct Pathogen Sequencing for Diagnosis of Lepromatous Leprosy and Cerebral Coenurosis |
| title_sort | modern solutions for ancient pathogens: direct pathogen sequencing for diagnosis of lepromatous leprosy and cerebral coenurosis |
| topic | Brief Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472670/ https://www.ncbi.nlm.nih.gov/pubmed/36119959 http://dx.doi.org/10.1093/ofid/ofac428 |
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