Evaluation of mTORC1 signaling in mouse atherosclerotic macrophages by flow cytometry and immunofluorescence

Previous studies have demonstrated that a high-protein diet leads to increased atherosclerosis in mice, and that this adverse effect is caused by activation of macrophage mTORC1 signaling. Here, we provide a detailed protocol for the evaluation of diet-induced mTORC1 signaling in plaque macrophages...

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Detalles Bibliográficos
Autores principales: Zhang, Xiangyu, Stitham, Jeremiah, Rodriguez-Velez, Astrid, Jeong, Se-Jin, Park, Arick, Yeh, Yu-Sheng, Kapoor, Divya, Mittendorfer, Bettina, Razani, Babak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474552/
https://www.ncbi.nlm.nih.gov/pubmed/36094885
http://dx.doi.org/10.1016/j.xpro.2022.101665

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474552/
https://www.ncbi.nlm.nih.gov/pubmed/36094885
http://dx.doi.org/10.1016/j.xpro.2022.101665

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