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Combined protein and nucleic acid staining in tissues with PANINI

We present here a detailed protocol for PANINI (protein and nucleic acid in situ imaging), a technique that enables the concurrent staining of protein and nucleic acids in archival tissue sections. PANINI utilizes an optimized antigen retrieval strategy that forgoes protease treatment while retainin...

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Detalles Bibliográficos
Autores principales: Deisher, Addison, Yeo, Yao Yu, Jiang, Sizun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9475321/
https://www.ncbi.nlm.nih.gov/pubmed/36097384
http://dx.doi.org/10.1016/j.xpro.2022.101663