Cargando…

Combined protein and nucleic acid staining in tissues with PANINI

We present here a detailed protocol for PANINI (protein and nucleic acid in situ imaging), a technique that enables the concurrent staining of protein and nucleic acids in archival tissue sections. PANINI utilizes an optimized antigen retrieval strategy that forgoes protease treatment while retainin...

Descripción completa

Detalles Bibliográficos
Autores principales:	Deisher, Addison, Yeo, Yao Yu, Jiang, Sizun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9475321/ https://www.ncbi.nlm.nih.gov/pubmed/36097384 http://dx.doi.org/10.1016/j.xpro.2022.101663

Ejemplares similares

PANINI: Pangenome Neighbour Identification for Bacterial Populations
por: Abudahab, Khalil, et al.
Publicado: (2018)

Physical Activity and Nutrition INfluences In ageing (PANINI): consortium mission statement
por: Whittaker, Anna C., et al.
Publicado: (2017)

Population assessment and habitat associations of the Visayan Hornbill (Penelopides panini) in Northwest Panay, Philippines
por: Mynott, Holly Isabelle, et al.
Publicado: (2021)

How to stain nucleic acids and proteins in Miller spreads
por: Zannino, Lorena, et al.
Publicado: (2022)

STUDIES ON NUCLEIC ACID METACHROMASY : II. Metachromatic and Orthochromatic Staining by Toluidine Blue of Nucleic Acids in Tissue Sections
por: Feder, Ned, et al.
Publicado: (1965)

THE USE OF BISMUTH AS AN ELECTRON STAIN FOR NUCLEIC ACIDS
por: Albersheim, Peter, et al.
Publicado: (1963)

Nucleic Acid Amplification Testing and Sequencing Combined with Acid-Fast Staining in Needle Biopsy Lung Tissues for the Diagnosis of Smear-Negative Pulmonary Tuberculosis
por: Jiang, Faming, et al.
Publicado: (2016)

Iron as a Stain for Nucleic Acids in Electron Microscopy
por: Bernstein, Maurice H.
Publicado: (1956)

METHODS FOR THE USE OF INDIUM AS AN ELECTRON STAIN FOR NUCLEIC ACIDS
por: Watson, Michael L., et al.
Publicado: (1961)

Protocol for whole-mount X-gal staining combined with tissue clearing in embryo and adult mouse using CUBIC
por: Watanabe-Takano, Haruko, et al.
Publicado: (2022)

Removing nucleic acids from nucleoid-associated proteins purified by affinity column
por: Vingadassalon, Audrey, et al.
Publicado: (2016)

Protocol for immunofluorescence staining of murine helminth-infected intestinal and lung tissues
por: El-Naccache, Darine W., et al.
Publicado: (2023)

PREFERENTIAL STAINING OF NUCLEIC ACID-CONTAINING STRUCTURES FOR ELECTRON MICROSCOPY
por: Huxley, H. E., et al.
Publicado: (1961)

THE REACTIVITY AND STAINING OF TISSUE PROTEINS WITH PHOSPHOTUNGSTIC ACID
por: Silverman, Lloyd, et al.
Publicado: (1969)

Combined protein and nucleic acid imaging reveals virus-dependent B cell and macrophage immunosuppression of tissue microenvironments
por: Jiang, Sizun, et al.
Publicado: (2022)

The Physical Activity and Nutritional INfluences in Ageing (PANINI) Toolkit: A Standardized Approach towards Physical Activity and Nutritional Assessment of Older Adults
por: Ramsey, Keenan A., et al.
Publicado: (2022)

Multiplex chromogenic immunohistochemistry to stain and analyze paraffin tissue sections from the mouse or human
por: Maiques, Oscar, et al.
Publicado: (2022)

METHYL GREEN-PYRONIN : I. BASIS OF SELECTIVE STAINING OF NUCLEIC ACIDS
por: Kurnick, N. B.
Publicado: (1950)

Getting two birds with one stone: Combining immunohistochemistry and Azan staining in animal morphology
por: Beckers, Patrick, et al.
Publicado: (2021)

Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice
por: Fra-Bido, Sigrid, et al.
Publicado: (2021)

Rapid fluorometric quantification of bacterial cells using Redsafe nucleic acid stain
por: Khalili, Ehsan, et al.
Publicado: (2015)

Intracranial delivery of antisense locked nucleic acids for silencing microRNAs in the mouse brain
por: Sharma, Nikhil, et al.
Publicado: (2022)

A simple and affordable kinetic assay of nucleic acids with SYBR Gold gel staining
por: Guillen, Danielle, et al.
Publicado: (2020)

Rapid Electrophoretic Staining and Destaining of Polyacrylamide Gels
por: Motojima, Fumihiro
Publicado: (2018)

An Inexpensive Staining Alternative for Gelatin Zymography Gels
por: Wechselberger, Christian, et al.
Publicado: (2019)

Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining
por: Atanga, Roger, et al.
Publicado: (2022)

Homebrew: Protocol for glassmilk-based nucleic-acid extraction for SARS-CoV-2 diagnostics
por: Page, Robert, et al.
Publicado: (2022)

Using gold nanobeacons as a theranostic technique to recognize, detect, and inhibit specific nucleic acids
por: Sousa, Diana Pacheco, et al.
Publicado: (2022)

Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks
por: Mason, Michael G., et al.
Publicado: (2020)

Preparation of Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing of nucleic acids
por: Wang, Yuqin, et al.
Publicado: (2021)

A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis
por: Hinton, James P., et al.
Publicado: (2019)

Mass cytometry staining for human bone marrow clinical samples
por: Hallisey, Margaret, et al.
Publicado: (2022)

Mouse tail skin wholemount staining and intravital calcium imaging
por: Xie, Yuhua, et al.
Publicado: (2022)

High-Throughput Mass Cytometry Staining for Immunophenotyping Clinical Samples
por: Thrash, Emily M., et al.
Publicado: (2020)

Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomes
por: Goldmann, Marie, et al.
Publicado: (2021)

A Simple, Rapid, and Effective Heparinase Protocol to Enable Nucleic Acid Study from Frozen Heparinized Plasma
por: Afruza, Rownock, et al.
Publicado: (2023)

Effect of not vortexing nasopharyngeal and throat swabs on SARS-CoV-2 nucleic acid detection: A pilot study
por: Kanji, Jamil N., et al.
Publicado: (2022)

Protocols for staining of bile canalicular and sinusoidal networks of human, mouse and pig livers, three-dimensional reconstruction and quantification of tissue microarchitecture by image processing and analysis
por: Hammad, Seddik, et al.
Publicado: (2014)

Production and purification of infectious diseases-associated tetramer for staining of CD8(+) T cells from PBMCs
por: Ye, Jiezhou, et al.
Publicado: (2022)

Whole-mount immunofluorescence staining of mesenchymal progenitors in murine plantaris muscle
por: Kurosawa, Tamaki, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_ioosi0q08iig8h4jhjulb6qf0h