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Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations

In the past 10 years, gene-editing and organoid culture have completely changed the process of biology. Congenital nervous system malformations are difficult to study due to their polygenic pathogenicity, the complexity of cellular and neural regions of the brain, and the dysregulation of specific n...

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Detalles Bibliográficos
Autores principales: Xiaoshuai, Li, Qiushi, Wang, Rui, Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478582/
https://www.ncbi.nlm.nih.gov/pubmed/36118578
http://dx.doi.org/10.3389/fbioe.2022.932936
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author Xiaoshuai, Li
Qiushi, Wang
Rui, Wang
author_facet Xiaoshuai, Li
Qiushi, Wang
Rui, Wang
author_sort Xiaoshuai, Li
collection PubMed
description In the past 10 years, gene-editing and organoid culture have completely changed the process of biology. Congenital nervous system malformations are difficult to study due to their polygenic pathogenicity, the complexity of cellular and neural regions of the brain, and the dysregulation of specific neurodevelopmental processes in humans. Therefore, the combined application of CRISPR-Cas9 in organoid models may provide a technical platform for studying organ development and congenital diseases. Here, we first summarize the occurrence of congenital neurological malformations and discuss the different modeling methods of congenital nervous system malformations. After that, it focuses on using organoid to model congenital nervous system malformations. Then we summarized the application of CRISPR-Cas9 in the organoid platform to study the pathogenesis and treatment strategies of congenital nervous system malformations and finally looked forward to the future.
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spelling pubmed-94785822022-09-17 Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations Xiaoshuai, Li Qiushi, Wang Rui, Wang Front Bioeng Biotechnol Bioengineering and Biotechnology In the past 10 years, gene-editing and organoid culture have completely changed the process of biology. Congenital nervous system malformations are difficult to study due to their polygenic pathogenicity, the complexity of cellular and neural regions of the brain, and the dysregulation of specific neurodevelopmental processes in humans. Therefore, the combined application of CRISPR-Cas9 in organoid models may provide a technical platform for studying organ development and congenital diseases. Here, we first summarize the occurrence of congenital neurological malformations and discuss the different modeling methods of congenital nervous system malformations. After that, it focuses on using organoid to model congenital nervous system malformations. Then we summarized the application of CRISPR-Cas9 in the organoid platform to study the pathogenesis and treatment strategies of congenital nervous system malformations and finally looked forward to the future. Frontiers Media S.A. 2022-09-02 /pmc/articles/PMC9478582/ /pubmed/36118578 http://dx.doi.org/10.3389/fbioe.2022.932936 Text en Copyright © 2022 Xiaoshuai, Qiushi and Rui. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Xiaoshuai, Li
Qiushi, Wang
Rui, Wang
Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
title Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
title_full Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
title_fullStr Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
title_full_unstemmed Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
title_short Advantages of CRISPR-Cas9 combined organoid model in the study of congenital nervous system malformations
title_sort advantages of crispr-cas9 combined organoid model in the study of congenital nervous system malformations
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478582/
https://www.ncbi.nlm.nih.gov/pubmed/36118578
http://dx.doi.org/10.3389/fbioe.2022.932936
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