Quality control recommendations for RNASeq using FFPE samples based on pre-sequencing lab metrics and post-sequencing bioinformatics metrics

BACKGROUND: Formalin-fixed, paraffin-embedded (FFPE) tissues have many advantages for identification of risk biomarkers, including wide availability and potential for extended follow-up endpoints. However, RNA derived from archival FFPE samples has limited quality. Here we identified parameters that...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yuanhang, Bhagwate, Aditya, Winham, Stacey J., Stephens, Melissa T., Harker, Brent W., McDonough, Samantha J., Stallings-Mann, Melody L., Heinzen, Ethan P., Vierkant, Robert A., Hoskin, Tanya L., Frost, Marlene H., Carter, Jodi M., Pfrender, Michael E., Littlepage, Laurie, Radisky, Derek C., Cunningham, Julie M., Degnim, Amy C., Wang, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479231/
https://www.ncbi.nlm.nih.gov/pubmed/36114500
http://dx.doi.org/10.1186/s12920-022-01355-0

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479231/
https://www.ncbi.nlm.nih.gov/pubmed/36114500
http://dx.doi.org/10.1186/s12920-022-01355-0

Ejemplares similares