Design and validation of recombinant protein standards for quantitative Western blot analysis of cannabinoid CB(1) receptor density in cell membranes: an alternative to radioligand binding methods

BACKGROUND: Replacement of radioligand binding assays with antibody-antigen interaction-based approaches for quantitative analysis of G protein-coupled receptor (GPCR) levels requires the use of purified protein standards containing the antigen. GPCRs in general and cannabinoid CB(1) receptor in par...

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Detalles Bibliográficos
Autores principales: Saumell-Esnaola, Miquel, Elejaga-Jimeno, Ainhoa, Echeazarra, Leyre, Borrega-Román, Leire, Barrondo, Sergio, López de Jesús, Maider, González-Burguera, Imanol, Gómez-Caballero, Alberto, Goicolea, María Aranzazu, Sallés, Joan, García del Caño, Gontzal
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479267/
https://www.ncbi.nlm.nih.gov/pubmed/36109736
http://dx.doi.org/10.1186/s12934-022-01914-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479267/
https://www.ncbi.nlm.nih.gov/pubmed/36109736
http://dx.doi.org/10.1186/s12934-022-01914-1

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