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Fluorescence quenching by high-power LEDs for highly sensitive fluorescence in situ hybridization

Recent technical advances have made fluorescent in situ hybridization (ISH) a pivotal method to analyze neural tissue. In a highly sensitive ISH, it is important to reduce tissue autofluorescence. We developed a photobleaching device using a light-emitting diode (LED) illuminator to quench autofluor...

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Detalles Bibliográficos
Autores principales: Tsuneoka, Yousuke, Atsumi, Yusuke, Makanae, Aki, Yashiro, Mitsuru, Funato, Hiromasa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9479452/
https://www.ncbi.nlm.nih.gov/pubmed/36117911
http://dx.doi.org/10.3389/fnmol.2022.976349