Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites

Here we present an approach that combines a clustered regularly interspaced short palindromic repeats (CRISPR) system that simultaneously targets hundreds of epigenetically diverse endogenous genomic sites with high-throughput sequencing to measure Cas9 dynamics and cellular responses at scale. This...

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Detalles Bibliográficos
Autores principales: Zou, Roger S., Marin-Gonzalez, Alberto, Liu, Yang, Liu, Hans B., Shen, Leo, Dveirin, Rachel K., Luo, Jay X. J., Kalhor, Reza, Ha, Taekjip
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481459/
https://www.ncbi.nlm.nih.gov/pubmed/36064968
http://dx.doi.org/10.1038/s41556-022-00975-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481459/
https://www.ncbi.nlm.nih.gov/pubmed/36064968
http://dx.doi.org/10.1038/s41556-022-00975-z

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