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Quantitative analysis of CRISPR/Cas9-mediated provirus deletion in blue egg layer chicken PGCs by digital PCR

Primordial germ cells (PGCs), the precursors of sperm and oocytes, pass on the genetic material to the next generation. The previously established culture system of chicken PGCs holds many possibilities for functional genomics studies and the rapid introduction of desired traits. Here, we establishe...

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Detalles Bibliográficos
Autores principales: Altgilbers, Stefanie, Dierks, Claudia, Klein, Sabine, Weigend, Steffen, Kues, Wilfried A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481566/
https://www.ncbi.nlm.nih.gov/pubmed/36114266
http://dx.doi.org/10.1038/s41598-022-19861-7