Cargando…

Quantitative analysis of CRISPR/Cas9-mediated provirus deletion in blue egg layer chicken PGCs by digital PCR

Primordial germ cells (PGCs), the precursors of sperm and oocytes, pass on the genetic material to the next generation. The previously established culture system of chicken PGCs holds many possibilities for functional genomics studies and the rapid introduction of desired traits. Here, we establishe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Altgilbers, Stefanie, Dierks, Claudia, Klein, Sabine, Weigend, Steffen, Kues, Wilfried A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481566/ https://www.ncbi.nlm.nih.gov/pubmed/36114266 http://dx.doi.org/10.1038/s41598-022-19861-7

Ejemplares similares

Cultivation and characterization of primordial germ cells from blue layer hybrids (Araucana crossbreeds) and generation of germline chimeric chickens
por: Altgilbers, Stefanie, et al.
Publicado: (2021)

Identification of chicken LOC420478 as Bucky ball equivalent and potential germ plasm organizer in birds
por: Klein, Sabine, et al.
Publicado: (2022)

Harnessing the CRISPR/Cas9 system to disrupt latent HIV-1 provirus
por: Ebina, Hirotaka, et al.
Publicado: (2013)

CRISPR/Cas9 Genome Editing to Disable the Latent HIV-1 Provirus
por: Panfil, Amanda R., et al.
Publicado: (2018)

Targeted Chromatinization and Repression of HIV-1 Provirus Transcription with Repurposed CRISPR/Cas9
por: Olson, Alex, et al.
Publicado: (2020)

CRISPR/Cas9-mediated targeted knock-in of large constructs using nocodazole and RNase HII
por: Eghbalsaied, Shahin, et al.
Publicado: (2023)

Transcriptome Sequencing and Comparative Analysis of Amphoteric ESCs and PGCs in Chicken (Gallus gallus)
por: Jin, Kai, et al.
Publicado: (2020)

Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens
por: Jiang, Zi-Qin, et al.
Publicado: (2020)

Linkage disequilibrium reveals different demographic history in egg laying chickens
por: Qanbari, Saber, et al.
Publicado: (2010)

Targeting and Understanding HIV Latency: The CRISPR System against the Provirus
por: Magro, Gloria, et al.
Publicado: (2021)

Insertion of sequences at the original provirus integration site of mouse ROSA26 locus using the CRISPR/Cas9 system
por: Quadros, Rolen M., et al.
Publicado: (2015)

Relationship between Bone Stability and Egg Production in Genetically Divergent Chicken Layer Lines
por: Jansen, Simon, et al.
Publicado: (2020)

Fate Decisions of Chicken Primordial Germ Cells (PGCs): Development, Integrity, Sex Determination, and Self-Renewal Mechanisms
por: Ichikawa, Kennosuke, et al.
Publicado: (2023)

A CRISPR/Cas9 guidance RNA screen platform for HIV provirus disruption and HIV/AIDS gene therapy in astrocytes
por: Huang, Zaohua, et al.
Publicado: (2017)

Transcriptomic profile of early zebrafish PGCs by single cell sequencing
por: Zhang, Xiaoyuan, et al.
Publicado: (2019)

Egg Production and Bone Stability of Local Chicken Breeds and Their Crosses Fed with Faba Beans
por: Nolte, Tanja, et al.
Publicado: (2020)

Three-dimensional culture of chicken primordial germ cells (cPGCs) in defined media containing the functional polymer FP003
por: Chen, Yi-Chen, et al.
Publicado: (2018)

C1EIP Functions as an Activator of ENO1 to Promote Chicken PGCs Formation via Inhibition of the Notch Signaling Pathway
por: Jin, Kai, et al.
Publicado: (2020)

Genotypic and Dietary Effects on Egg Quality of Local Chicken Breeds and Their Crosses Fed with Faba Beans
por: Nolte, Tanja, et al.
Publicado: (2021)

CRISPR/Cas9-Based Deletion of SpvB Gene From Salmonella gallinarum Leads to Loss of Virulence in Chicken
por: Basit, Abdul, et al.
Publicado: (2022)

Correction: Jansen, S., et al. Relationship between Bone Stability and Egg Production in Genetically Divergent Chicken Layer Lines. Animals 2020, 10, 850
por: Jansen, Simon, et al.
Publicado: (2020)

Chromosomal deletions and inversions mediated by TALENs and CRISPR/Cas in zebrafish
por: Xiao, An, et al.
Publicado: (2013)

CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
por: Amorim, João Pedro, et al.
Publicado: (2020)

Engineering large-scale chromosomal deletions by CRISPR-Cas9
por: Eleveld, Thomas F, et al.
Publicado: (2021)

Targeted mutagenesis in chicken using CRISPR/Cas9 system
por: Oishi, Isao, et al.
Publicado: (2016)

Epidemiologic Profile of Hypertension in Northern Iranian Population: The PERSIAN Guilan Cohort Study (PGCS)
por: Naghipour, Mohammadreza, et al.
Publicado: (2021)

Accession-Dependent CBF Gene Deletion by CRISPR/Cas System in Arabidopsis
por: Cho, Sungkyung, et al.
Publicado: (2017)

CRISPR/Cas9 Methodology for the Generation of Knockout Deletions in Caenorhabditis elegans
por: Au, Vinci, et al.
Publicado: (2018)

CRISPR/Cas9-Mediated Deletion of Large Genomic Fragments in Soybean
por: Cai, Yupeng, et al.
Publicado: (2018)

Large-scale genomic deletions mediated by CRISPR/Cas9 system
por: Song, Yuning, et al.
Publicado: (2017)

CRISPR/Cas9 deletion of ORMDLs reveals complexity in sphingolipid metabolism
por: Green, Christopher D., et al.
Publicado: (2021)

Recyclable CRISPR/Cas9 mediated gene disruption and deletions in Histoplasma
por: Joehnk, Bastian, et al.
Publicado: (2023)

The SYNBREED chicken diversity panel: a global resource to assess chicken diversity at high genomic resolution
por: Malomane, Dorcus Kholofelo, et al.
Publicado: (2019)

Somatic mutations in mitochondria: the chicken or the egg?
por: Ospelt, Caroline, et al.
Publicado: (2005)

The Role of Egg Production in the Etiology of Keel Bone Damage in Laying Hens
por: Eusemann, Beryl Katharina, et al.
Publicado: (2020)

The landscape of mitochondrial small non-coding RNAs in the PGCs of male mice, spermatogonia, gametes and in zygotes
por: Larriba, Eduardo, et al.
Publicado: (2018)

Population Genomic Analyses Based on 1 Million SNPs in Commercial Egg Layers
por: Gholami, Mahmood, et al.
Publicado: (2014)

CRISPR-Cas12a–assisted PCR tagging of mammalian genes
por: Fueller, Julia, et al.
Publicado: (2020)

Large Genomic Fragment Deletions and Insertions in Mouse Using CRISPR/Cas9
por: Zhang, Luqing, et al.
Publicado: (2015)

CRISPR-Cas9 mediated gene deletions in lager yeast Saccharomyces pastorianus
por: de Vries, Arthur R. Gorter, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_oi2j361lsm93nfieicntht7th7