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Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals

Transgenic animals expressing fluorescent proteins are widely used to label specific cells and proteins. By using a split Cre recombinase fused with mCherry-binding nanobodies or designed ankyrin repeat proteins, we created Cre recombinase dependent on red fluorescent protein (RFP) (Cre-DOR). Functi...

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Detalles Bibliográficos
Autores principales: Inutsuka, Ayumu, Maejima, Sho, Mizoguchi, Hiroyuki, Kaneko, Ryosuke, Nomura, Rei, Takanami, Keiko, Sakamoto, Hirotaka, Onaka, Tatsushi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9481622/
https://www.ncbi.nlm.nih.gov/pubmed/36114373
http://dx.doi.org/10.1038/s42003-022-03944-2