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Determination of reference genes as a quantitative standard for gene expression analysis in mouse mesangial cells stimulated with TGF-β

Reverse transcription-quantitative polymerase chain reaction (RT-PCR) is the gold standard technique for gene expression analysis, but the choice of quantitative reference genes (housekeeping genes, HKG) remains challenging. Identify the best HKG is essential for estimating the expression level of t...

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Detalles Bibliográficos
Autores principales: dos Santos Bronel, Bruno Aristides, Anauate, Ana Carolina, Maquigussa, Edgar, Boim, Mirian Aparecida, da Silva Novaes, Antônio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9482652/
https://www.ncbi.nlm.nih.gov/pubmed/36115882
http://dx.doi.org/10.1038/s41598-022-19548-z