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Protocol for multiplex fluorescent immunohistochemistry in free-floating rodent brain tissues

Identifying multiple proteins within the same tissue allows for assessing protein colocalization, is cost effective, and maximizes efficiency. Here, we describe a protocol for multiplex immunolabeling of proteins in free-floating rodent brain sections. As opposed to slide-mounted immunohistochemistr...

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Detalles Bibliográficos
Autores principales: Kelly, Aubrey M., Fricker, Brandon A., Wallace, Kelly J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9483644/
https://www.ncbi.nlm.nih.gov/pubmed/36107743
http://dx.doi.org/10.1016/j.xpro.2022.101672