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Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations

BACKGROUND: O(2)-tolerant [NiFe]-hydrogenases offer tremendous potential for applications in H(2)-based technology. As these metalloenzymes undergo a complicated maturation process that requires a dedicated set of multiple accessory proteins, their heterologous production is challenging, thus hinder...

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Detalles Bibliográficos
Autores principales: Fan, Qin, Waldburger, Saskia, Neubauer, Peter, Riedel, Sebastian L., Gimpel, Matthias
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484157/
https://www.ncbi.nlm.nih.gov/pubmed/36123684
http://dx.doi.org/10.1186/s12934-022-01919-w