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Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations
BACKGROUND: O(2)-tolerant [NiFe]-hydrogenases offer tremendous potential for applications in H(2)-based technology. As these metalloenzymes undergo a complicated maturation process that requires a dedicated set of multiple accessory proteins, their heterologous production is challenging, thus hinder...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484157/ https://www.ncbi.nlm.nih.gov/pubmed/36123684 http://dx.doi.org/10.1186/s12934-022-01919-w |