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Implementation of a high cell density fed-batch for heterologous production of active [NiFe]-hydrogenase in Escherichia coli bioreactor cultivations

BACKGROUND: O(2)-tolerant [NiFe]-hydrogenases offer tremendous potential for applications in H(2)-based technology. As these metalloenzymes undergo a complicated maturation process that requires a dedicated set of multiple accessory proteins, their heterologous production is challenging, thus hinder...

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Detalles Bibliográficos
Autores principales:	Fan, Qin, Waldburger, Saskia, Neubauer, Peter, Riedel, Sebastian L., Gimpel, Matthias
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2022
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9484157/ https://www.ncbi.nlm.nih.gov/pubmed/36123684 http://dx.doi.org/10.1186/s12934-022-01919-w

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