Using mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity

Numerous approaches have been developed to isolate microglia from the brain, but procedures using enzymatic dissociation at 37°C can introduce drastic transcriptomic changes and confound results from gene expression assays. Here, we present an optimized protocol for microglia isolation using mechani...

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Detalles Bibliográficos
Autores principales: Herron, Shawn, Delpech, Jean-Christophe, Madore, Charlotte, Ikezu, Tsuneya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485281/
https://www.ncbi.nlm.nih.gov/pubmed/36107747
http://dx.doi.org/10.1016/j.xpro.2022.101670

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485281/
https://www.ncbi.nlm.nih.gov/pubmed/36107747
http://dx.doi.org/10.1016/j.xpro.2022.101670

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