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Using mechanical homogenization to isolate microglia from mouse brain tissue to preserve transcriptomic integrity
Numerous approaches have been developed to isolate microglia from the brain, but procedures using enzymatic dissociation at 37°C can introduce drastic transcriptomic changes and confound results from gene expression assays. Here, we present an optimized protocol for microglia isolation using mechani...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485281/ https://www.ncbi.nlm.nih.gov/pubmed/36107747 http://dx.doi.org/10.1016/j.xpro.2022.101670 |