Optimized immunofluorescence staining protocol for identifying resident mesenchymal stem cells in bone using LacZ transgenic mice

Glioma-associated oncogene homolog 1 (Gli1) marks a subpopulation of endogenous mesenchymal stem cells (MSCs) characterized by perivascular location. Here, we present an optimized immunofluorescence staining protocol to identify resident Gli1(+) MSCs in fixed/frozen bone sections from LacZ transgeni...

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Detalles Bibliográficos
Autores principales: Zheng, Chen-Xi, Chen, Ji, Tian, Jiong-Yi, Huang, Xiao-Yao, Jin, Yan, Sui, Bing-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485525/
https://www.ncbi.nlm.nih.gov/pubmed/36107746
http://dx.doi.org/10.1016/j.xpro.2022.101674

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9485525/
https://www.ncbi.nlm.nih.gov/pubmed/36107746
http://dx.doi.org/10.1016/j.xpro.2022.101674

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