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Inducible CRISPR/Cas9 Allows for Multiplexed and Rapidly Segregated Single-Target Genome Editing in Synechocystis Sp. PCC 6803

[Image: see text] Establishing various synthetic biology tools is crucial for the development of cyanobacteria for biotechnology use, especially tools that allow for precise and markerless genome editing in a time-efficient manner. Here, we describe a riboswitch-inducible CRISPR/Cas9 system, contain...

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Detalles Bibliográficos
Autores principales: Cengic, Ivana, Cañadas, Inés C., Minton, Nigel P., Hudson, Elton P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486961/
https://www.ncbi.nlm.nih.gov/pubmed/35969224
http://dx.doi.org/10.1021/acssynbio.2c00375