Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation

[Image: see text] The physical stability of bacterial chromosomes is important for their in vitro manipulation, while genetic stability is important in vivo. However, extracted naked chromosomes in the open circular form are fragile due to nicks and gaps. Using a nick/gap repair and negative superco...

Descripción completa

Detalles Bibliográficos
Autores principales: Fujita, Hironobu, Osaku, Ayane, Sakane, Yuto, Yoshida, Koki, Yamada, Kayoko, Nara, Seia, Mukai, Takahito, Su’etsugu, Masayuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486964/
https://www.ncbi.nlm.nih.gov/pubmed/35998348
http://dx.doi.org/10.1021/acssynbio.2c00353

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486964/
https://www.ncbi.nlm.nih.gov/pubmed/35998348
http://dx.doi.org/10.1021/acssynbio.2c00353

Ejemplares similares