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Enzymatic Supercoiling of Bacterial Chromosomes Facilitates Genome Manipulation
[Image: see text] The physical stability of bacterial chromosomes is important for their in vitro manipulation, while genetic stability is important in vivo. However, extracted naked chromosomes in the open circular form are fragile due to nicks and gaps. Using a nick/gap repair and negative superco...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Chemical Society
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9486964/ https://www.ncbi.nlm.nih.gov/pubmed/35998348 http://dx.doi.org/10.1021/acssynbio.2c00353 |