Protocol to evaluate cell lineage stability of mouse natural and induced regulatory T cells using bisulfite sequencing

The establishment of regulatory T cells (Treg)-specific demethylation regions (TSDRs) is essential for the Treg-lineage stability. Here, we present a protocol using bisulfite sequencing to assess Treg-lineage stability. The protocol describes the isolation of lymphocytes and DNA extraction, followed...

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Detalles Bibliográficos
Autores principales: Arai, Masaya, Fukuda, Aine, Morimoto, Reo, Nakamura, Yamami, Ci, Zhaohong, Sakaguchi, Shimon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489535/
https://www.ncbi.nlm.nih.gov/pubmed/36121747
http://dx.doi.org/10.1016/j.xpro.2022.101694

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489535/
https://www.ncbi.nlm.nih.gov/pubmed/36121747
http://dx.doi.org/10.1016/j.xpro.2022.101694

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