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Protocol to evaluate cell lineage stability of mouse natural and induced regulatory T cells using bisulfite sequencing
The establishment of regulatory T cells (Treg)-specific demethylation regions (TSDRs) is essential for the Treg-lineage stability. Here, we present a protocol using bisulfite sequencing to assess Treg-lineage stability. The protocol describes the isolation of lymphocytes and DNA extraction, followed...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9489535/ https://www.ncbi.nlm.nih.gov/pubmed/36121747 http://dx.doi.org/10.1016/j.xpro.2022.101694 |