Protocol to detect RNAs from tissue sections in mice using Y-branched probe insitu hybridization

Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections. We provide steps for the synthesis and quantification of preamplifier probe using nickase. We also detail the preparation of ti...

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Detalles Bibliográficos
Autores principales: Wu, Yunming, Yu, C. Ron
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490196/
https://www.ncbi.nlm.nih.gov/pubmed/36115025
http://dx.doi.org/10.1016/j.xpro.2022.101686

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490196/
https://www.ncbi.nlm.nih.gov/pubmed/36115025
http://dx.doi.org/10.1016/j.xpro.2022.101686

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