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Protocol to detect RNAs from tissue sections in mice using Y-branched probe insitu hybridization
Here, we describe a fluorescent in situ hybridization protocol named Yn-situ, standing for Y-branched probe in situ hybridization, to detect RNAs from mice tissue sections. We provide steps for the synthesis and quantification of preamplifier probe using nickase. We also detail the preparation of ti...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490196/ https://www.ncbi.nlm.nih.gov/pubmed/36115025 http://dx.doi.org/10.1016/j.xpro.2022.101686 |