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Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection
Here, we present a protocol to analyze the T cell profiles of the neonatal ovine lung during respiratory syncytial virus (RSV) infection. The protocol delivers standardized multiparameter flow cytometry (FCM) analysis of CD4(+), CD8(+), regulatory, and γδ T cells isolated from lung, lymph nodes, and...
Autores principales: | Démoulins, Thomas, Brügger, Melanie, Zumkehr, Beatrice, Oliveira Esteves, Blandina I., Ruggli, Nicolas, Alves, Marco P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490204/ https://www.ncbi.nlm.nih.gov/pubmed/36116074 http://dx.doi.org/10.1016/j.xpro.2022.101688 |
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