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Multiparameter flow cytometry assay to analyze the pulmonary T cell profiles in the ovine model of respiratory syncytial virus infection

Here, we present a protocol to analyze the T cell profiles of the neonatal ovine lung during respiratory syncytial virus (RSV) infection. The protocol delivers standardized multiparameter flow cytometry (FCM) analysis of CD4(+), CD8(+), regulatory, and γδ T cells isolated from lung, lymph nodes, and...

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Detalles Bibliográficos
Autores principales: Démoulins, Thomas, Brügger, Melanie, Zumkehr, Beatrice, Oliveira Esteves, Blandina I., Ruggli, Nicolas, Alves, Marco P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9490204/
https://www.ncbi.nlm.nih.gov/pubmed/36116074
http://dx.doi.org/10.1016/j.xpro.2022.101688