Efficient assembly of long DNA fragments and multiple genes with improved nickase‐based cloning and Cre/ loxP recombination

Functional genomics, synthetic biology and metabolic engineering require efficient tools to deliver long DNA fragments or multiple gene constructs. Although numerous DNA assembly methods exist, most are complicated, time‐consuming and expensive. Here, we developed a simple and flexible strategy, uni...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Yanchang, Han, Jingluan, Tan, Jiantao, Yang, Yaqian, Li, Shuangchun, Gou, Yajun, Luo, Yuyu, Li, Tie, Xiao, Wenyu, Xue, Yang, Hao, Yu, Xie, Xianrong, Liu, Yao‐Guang, Zhu, Qinlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491458/
https://www.ncbi.nlm.nih.gov/pubmed/35767383
http://dx.doi.org/10.1111/pbi.13882

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9491458/
https://www.ncbi.nlm.nih.gov/pubmed/35767383
http://dx.doi.org/10.1111/pbi.13882

Ejemplares similares