Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )

Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated st...

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Autores principales: Rydell, Gustaf E, Prakash, Kasthuri, Larsson, Simon B, Skoglund, Catarina, Ringlander, Johan, Andersson, Maria E, Castedal, Maria, Norder, Heléne, Lindh, Magnus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492311/
https://www.ncbi.nlm.nih.gov/pubmed/34534318
http://dx.doi.org/10.1093/infdis/jiab469
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author Rydell, Gustaf E
Prakash, Kasthuri
Larsson, Simon B
Skoglund, Catarina
Ringlander, Johan
Andersson, Maria E
Castedal, Maria
Norder, Heléne
Lindh, Magnus
author_facet Rydell, Gustaf E
Prakash, Kasthuri
Larsson, Simon B
Skoglund, Catarina
Ringlander, Johan
Andersson, Maria E
Castedal, Maria
Norder, Heléne
Lindh, Magnus
author_sort Rydell, Gustaf E
collection PubMed
description Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated strongly when replication was high, supporting that transcription was from covalently closed circular DNA (cccDNA). By contrast, enhanced expression of S RNA relative to cccDNA and core RNA in patients with medium-high or low replication supports that HBV surface antigen (HBsAg) can be expressed mainly from integrated HBV DNA in such patients.
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spelling pubmed-94923112022-09-22 Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( ) Rydell, Gustaf E Prakash, Kasthuri Larsson, Simon B Skoglund, Catarina Ringlander, Johan Andersson, Maria E Castedal, Maria Norder, Heléne Lindh, Magnus J Infect Dis Brief Report Hepatitis B virus (HBV) DNA and RNA were quantified by digital PCR assays in 20–30 tissue pieces from each of 4 liver explants with cirrhosis caused by HBV. The within-patient variability of HBV RNA levels between pieces was up to a 1000-fold. Core RNA and S RNA levels were similar and correlated strongly when replication was high, supporting that transcription was from covalently closed circular DNA (cccDNA). By contrast, enhanced expression of S RNA relative to cccDNA and core RNA in patients with medium-high or low replication supports that HBV surface antigen (HBsAg) can be expressed mainly from integrated HBV DNA in such patients. Oxford University Press 2021-09-17 /pmc/articles/PMC9492311/ /pubmed/34534318 http://dx.doi.org/10.1093/infdis/jiab469 Text en © The Author(s) 2022. Published by Oxford University Press for the Infectious Diseases Society of America. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Brief Report
Rydell, Gustaf E
Prakash, Kasthuri
Larsson, Simon B
Skoglund, Catarina
Ringlander, Johan
Andersson, Maria E
Castedal, Maria
Norder, Heléne
Lindh, Magnus
Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )
title Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )
title_full Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )
title_fullStr Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )
title_full_unstemmed Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )
title_short Quantification of Viral RNA in Multiple Pieces of Explant Liver Tissue Shows Distinct Focal Differences in Hepatitis B Infection( )
title_sort quantification of viral rna in multiple pieces of explant liver tissue shows distinct focal differences in hepatitis b infection( )
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9492311/
https://www.ncbi.nlm.nih.gov/pubmed/34534318
http://dx.doi.org/10.1093/infdis/jiab469
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