Reconstructing cancer phylogenies using Pairtree, a clone tree reconstruction algorithm

Pairtree is a clone tree reconstruction algorithm that uses somatic point mutations to build clone trees describing the evolutionary history of individual cancers. Using the Pairtree software package, we describe steps to preprocess somatic mutation data, cluster mutations into subclones, search for...

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Detalles Bibliográficos
Autores principales: Kulman, Ethan, Wintersinger, Jeff, Morris, Quaid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9494285/
https://www.ncbi.nlm.nih.gov/pubmed/36129821
http://dx.doi.org/10.1016/j.xpro.2022.101706
Descripción
Sumario:Pairtree is a clone tree reconstruction algorithm that uses somatic point mutations to build clone trees describing the evolutionary history of individual cancers. Using the Pairtree software package, we describe steps to preprocess somatic mutation data, cluster mutations into subclones, search for clone trees, and visualize clone trees. Pairtree builds clone trees using up to 100 samples from a single cancer with at least 30 subclonal populations. For complete details on the use and execution of this protocol, please refer to Wintersinger et al. (2022).

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