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The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera

Rat basophilic leukaemia (RBL) cells have been used for decades as a model of high-affinity Immunoglobulin E (IgE) receptor (FcεRI) signalling. Here, we describe the generation and use of huNPY-mRFP, a new humanised fluorescent IgE reporter cell line. Fusion of Neuropeptide Y (NPY) with monomeric re...

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Detalles Bibliográficos
Autores principales: Prakash, Prema S., Barwary, Nafal J. S., Weber, Michael H. W., Wan, Daniel, Conejeros, Iván, Pereira Moreira, Bernardo, Alharbi, Waleed S., van Hellemond, Jaap J., Akinwale, Jude, Falcone, Franco H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9497870/
https://www.ncbi.nlm.nih.gov/pubmed/36140465
http://dx.doi.org/10.3390/diagnostics12092063