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Simultaneous SARS-CoV-2 Genome Sequencing of 384 Samples on an Illumina MiSeq Instrument through Protocol Optimization

In the present study, we propose a high-throughput sequencing protocol using aNextera XT Library DNA kit on an Illumina MiSeq instrument. We made major modifications to this library preparation in order to multiplex 384 samples in a single Illumina flow cell. To validate our protocol, we compared th...

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Detalles Bibliográficos
Autores principales: Papa Mze, Nasserdine, Beye, Mamadou, Kacel, Idir, Tola, Raphael, Basco, Leonardo, Bogreau, Hervé, Colson, Philippe, Fournier, Pierre-Edouard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9498777/
https://www.ncbi.nlm.nih.gov/pubmed/36140815
http://dx.doi.org/10.3390/genes13091648