Single-Step Genome Editing of Small Ruminant Embryos by Electroporation

We investigated the possibility of single-step genome editing in small ruminants by CRISPR-Cas9 zygote electroporation. We targeted SOCS2 and PDX1 in sheep embryos and OTX2 in goat embryos, utilizing a dual sgRNA approach. Gene editing efficiency was compared between microinjection and three differe...

Descripción completa

Detalles Bibliográficos
Autores principales: Mahdi, Ahmed K., Medrano, Juan F., Ross, Pablo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499182/
https://www.ncbi.nlm.nih.gov/pubmed/36142132
http://dx.doi.org/10.3390/ijms231810218

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9499182/
https://www.ncbi.nlm.nih.gov/pubmed/36142132
http://dx.doi.org/10.3390/ijms231810218

Ejemplares similares