A Label-Free Fluorescence Aptasensor Based on G-Quadruplex/Thioflavin T Complex for the Detection of Trypsin

A novel, label-free fluorescent assay has been developed for the detection of trypsin by using thioflavin T as a fluorescent probe. A specific DNA aptamer can be combined by adding cytochrome c. Trypsin hydrolyzes the cytochrome c into small peptide fragments, exposing the G-quadruplex part of DNA a...

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Detalles Bibliográficos
Autores principales: Gu, Pan, Lu, Yangfan, Li, Shanni, Ma, Changbei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503660/
https://www.ncbi.nlm.nih.gov/pubmed/36144829
http://dx.doi.org/10.3390/molecules27186093

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503660/
https://www.ncbi.nlm.nih.gov/pubmed/36144829
http://dx.doi.org/10.3390/molecules27186093

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