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Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs

Infection of pigs with the African swine fever virus (ASFV) leads to a devastating hemorrhagic disease with a high mortality of up to 100%. In this study, a CD2v gene deletion was introduced to a genotype IX virus from East Africa, ASFV-Kenya-IX-1033 (ASFV-Kenya-IX-1033-∆CD2v), to investigate whethe...

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Autores principales: Hemmink, Johanneke D., Khazalwa, Emmanuel M., Abkallo, Hussein M., Oduor, Bernard, Khayumbi, Jeremiah, Svitek, Nicholas, Henson, Sonal P., Blome, Sandra, Keil, Günther, Bishop, Richard P., Steinaa, Lucilla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503863/
https://www.ncbi.nlm.nih.gov/pubmed/36146726
http://dx.doi.org/10.3390/v14091917
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author Hemmink, Johanneke D.
Khazalwa, Emmanuel M.
Abkallo, Hussein M.
Oduor, Bernard
Khayumbi, Jeremiah
Svitek, Nicholas
Henson, Sonal P.
Blome, Sandra
Keil, Günther
Bishop, Richard P.
Steinaa, Lucilla
author_facet Hemmink, Johanneke D.
Khazalwa, Emmanuel M.
Abkallo, Hussein M.
Oduor, Bernard
Khayumbi, Jeremiah
Svitek, Nicholas
Henson, Sonal P.
Blome, Sandra
Keil, Günther
Bishop, Richard P.
Steinaa, Lucilla
author_sort Hemmink, Johanneke D.
collection PubMed
description Infection of pigs with the African swine fever virus (ASFV) leads to a devastating hemorrhagic disease with a high mortality of up to 100%. In this study, a CD2v gene deletion was introduced to a genotype IX virus from East Africa, ASFV-Kenya-IX-1033 (ASFV-Kenya-IX-1033-∆CD2v), to investigate whether this deletion led to reduced virulence in domestic pigs and to see if inoculation with this LA-ASFV could induce protective immunity against parental virus challenge. All pigs inoculated with ASFV-Kenya-IX-1033-ΔCD2v survived inoculation but presented with fever, reduced appetite and lethargy. ASFV genomic copies were detected in only one animal at one time point. Seven out of eight animals survived subsequent challenge with the pathogenic parental strain (87.5%) but had mild to moderate clinical symptoms and had a gross pathology compatible with chronic ASFV infection. All mock-immunised animals developed acute ASF upon challenge with ASFV-Kenya-IX-1033 and were euthanised upon meeting the humane endpoint criteria. ASFV genome copy numbers after challenge were similar in the two groups. ASFV-Kenya-IX-1033-∆CD2v is therefore a useful tool to investigate the development of immunity to ASFV genotype IX, but safety concerns preclude its use as a candidate vaccine without further attenuation.
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spelling pubmed-95038632022-09-24 Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs Hemmink, Johanneke D. Khazalwa, Emmanuel M. Abkallo, Hussein M. Oduor, Bernard Khayumbi, Jeremiah Svitek, Nicholas Henson, Sonal P. Blome, Sandra Keil, Günther Bishop, Richard P. Steinaa, Lucilla Viruses Article Infection of pigs with the African swine fever virus (ASFV) leads to a devastating hemorrhagic disease with a high mortality of up to 100%. In this study, a CD2v gene deletion was introduced to a genotype IX virus from East Africa, ASFV-Kenya-IX-1033 (ASFV-Kenya-IX-1033-∆CD2v), to investigate whether this deletion led to reduced virulence in domestic pigs and to see if inoculation with this LA-ASFV could induce protective immunity against parental virus challenge. All pigs inoculated with ASFV-Kenya-IX-1033-ΔCD2v survived inoculation but presented with fever, reduced appetite and lethargy. ASFV genomic copies were detected in only one animal at one time point. Seven out of eight animals survived subsequent challenge with the pathogenic parental strain (87.5%) but had mild to moderate clinical symptoms and had a gross pathology compatible with chronic ASFV infection. All mock-immunised animals developed acute ASF upon challenge with ASFV-Kenya-IX-1033 and were euthanised upon meeting the humane endpoint criteria. ASFV genome copy numbers after challenge were similar in the two groups. ASFV-Kenya-IX-1033-∆CD2v is therefore a useful tool to investigate the development of immunity to ASFV genotype IX, but safety concerns preclude its use as a candidate vaccine without further attenuation. MDPI 2022-08-30 /pmc/articles/PMC9503863/ /pubmed/36146726 http://dx.doi.org/10.3390/v14091917 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hemmink, Johanneke D.
Khazalwa, Emmanuel M.
Abkallo, Hussein M.
Oduor, Bernard
Khayumbi, Jeremiah
Svitek, Nicholas
Henson, Sonal P.
Blome, Sandra
Keil, Günther
Bishop, Richard P.
Steinaa, Lucilla
Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs
title Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs
title_full Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs
title_fullStr Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs
title_full_unstemmed Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs
title_short Deletion of the CD2v Gene from the Genome of ASFV-Kenya-IX-1033 Partially Reduces Virulence and Induces Protection in Pigs
title_sort deletion of the cd2v gene from the genome of asfv-kenya-ix-1033 partially reduces virulence and induces protection in pigs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9503863/
https://www.ncbi.nlm.nih.gov/pubmed/36146726
http://dx.doi.org/10.3390/v14091917
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